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Nucleiwerestained (darkblue)with afluorescent mol-eculethat intercalates into the DNAdouble helix. Inthe stretchedstate {top), the thin and thick filaments do not interact;the Hand Ibandsarevery wide. A lower end of theconfidence interval can then be taken as an estimate of anupper safe dose of the chemical, in the absence of otheravailable information.

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Inthis context, the term nucleusmeans a clusteror group ofneuronal cell bodies plus fibers and neuroglia.

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